Laboratory for Radiobiology and translational Radiation Oncology

The Translational Radiooncology and Radiobiology Research Laboratory at the Charité –Berlin, is a part of the Department of Radiooncology and Radiotherapy. The focus of attention is the molecular characterization and the analysis of resistance mechanisms of tumors in the head and neck region.

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  • Laboratory for Radiobiology and translational Radiation Oncology.

Radiooncological research

The analysis of preclinical cell line models provides data for an improved understanding of the mechanisms of cancer. For this, observations from cell line based experiments are examined on patient samples in the context of clinical trials that are supervised by the department of radiooncolgy and radiotherapy.

To achieve this goal, we are:

  • working together with physicians of all three Charité-Campi in order to get as many patient samples as possible for our studies; in more detail we cooperate with the mouth-maxillofacial surgery, department of Otorhinolaryngology and clinics for hematology and oncology
  • establish patient-derived preclinical models in order to improve understanding of tumor biology
  • using state-of-the-art technologies (Next-generation-sequencing, 3D cell culture…)
  • establishing and optimizing liquid biopsy in order to develop a novel diagnostic tool for monitoring therapy response and early detection of tumorprogression

In order to predict therapy response, the analysis of the tumors` molecular profile can facilitate and support personalized treatment decision. In addition, these data enable the identification of promising, novel molecular biomarkers, which can be used either as diagnostic marker or as new target for drugs.

 

Methods

In order to ensure a successful implementation of the diverse projects, a broad methodological spectrum was established and expanded in the laboratory:

cell culture technology:

  • 3D cell culture of patient-derived organoid-like tumor spheres (PDOTS)
  • Colony formation assay
  • Cell viability assay
  • Radiation experiments using an X-ray irradiaton
  • drug testing
  • NEW: CRISPR/Cas9 technology

immunological methods:

  • flow cytometry (membrane-bound, intracellular)
  • imaging flow cytometry
  • fluorescence microscopy

molecular biological und protein biochemical methods:

  • digital droplet PCR (ddPCR)
  • real-time PCR
  • PCR
  • Next-Generation-Sequencing
  • Western Blot-Analysis